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From Faith Pring
Scientists at The University of Nottingham have demonstrated for the first time that it is possible to selectively sequence fragments of DNA in real time, greatly reducing the time needed to analyse biological samples.
A paper published today in the academic journal Nature Methods describes a novel technique for highly selective DNA sequencing, called ‘Read Until’. The method, used with real-time nanopore sequencing, enables the user to analyse only DNA strands that contain pre-determined signatures of interest.
Dr Matt Loose, of the Cell and Developmental Biology Research Group in the University’s School of Life Sciences, has been working with the MinION, a new portable DNA sequencing technology produced by biotech company Oxford Nanopore Technologies (www.nanoporetech.com). All sequencing was carried out at The University of Nottingham Next Generation Sequencing Facility, DeepSeq.
